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KMID : 0980220140140010041
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Journal of the Korean Dental Society of Anesthesiology
2014 Volume.14 No. 1 p.41 ~ p.47
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The Effect of Propofol on Hypoxic damaged-HaCaT Cells
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Park Chang-Hoon
Kwak Jin-Won
Park Bong-Soo
Kim Yong-Ho
Kim Yong-Deok
Yoon Ji-Uk
Yoon Ji-Young
Kim Cheul-Hong
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Abstract
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Background: Autophagy is a self-eating process that is important for balancing sources of energy at critical times in development and in response stress. Autophagy also plays a protective role in removing clearing damaged intracellular organelles and aggregated proteins as well as eliminating intracellular pathogens. The purpose of the present study was to examine the protective effect of propofol against hypoxic damage using keratinocytes.
Methods: Human keratinocytes (HaCaT cells) were obtained from the American Type Culture Collection. Propofol which were made by dissolving them in DMSO were kept frozen at -4℃ until use. The stock was diluted to their concentration with DMEM when needed. Prior to propofol treatment cells were grown to about 80% confluence and then exposed to propofol at different concentrations (0, 25, 50, 75, 100 μM) for 2 h pretreatment. Cell viability was measured using a quantitative colorimetric assay with thiazolyl blue tetrazolium bromide (MTT assay), and fluorescence microscopy and western blot analysis were used for evaluation of autophagy processes.
Results: The viability of propofol-treated HaCaT cells was increased in a dose-dependent manner. Propofol did not show any significant toxic effect on the HaCaT cells. The autophagy inhibitor, 3-methyladenine, reduced cell viability of hypoxia-injured HaCat cells. Fluorescence microscopy and western blot analysis showed propofol induce autophagy pathway signals.
Conclusions: Propofol enhanced viability of hypoxia-injured HaCaT cells and we suggest propofol has cellular protective effects by autophagy signal pathway activation.
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KEYWORD
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Autophagy, Hacat cell, Hypoxic damage, Propofol
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